The purpose of this study undertaken 2016-2017 was to verify a number of administrative-district-level baseline trachoma prevalence estimates from earlier surveys in Zambia. The study considered 32 population-based prevalence surveys covering 47 administrative districts. The results concluded that trachoma is a public health problem in some parts of Zambia. The Ministry of Health will continue to partner with stakeholders to implement the multi-sectoral SAFE strategy. Furthermore, consideration should be given to re-surveying other suspected-endemic administrative districts in which surveys using older methodologies returned prevalence estimates of trachomatous inflammation—follicular ?5%.