The purpose of this study undertaken in 2014 was to determine the prevalence of trachoma in each of the 25 local government areas (LGAs) of Niger State, Nigeria. A population-based prevalence survey was conducted in each LGA according to the methodology of the Global Trachoma Mapping Project. The study concluded that trachoma is generally hypoendemic, with the prevalence of active trachoma (TF) being less than the WHO elimination threshold of 5% in all but two LGAs. In these two LGAs mass distribution of azithromycin, together with facial cleanliness and environmental improvement is required. The prevalence of trichiasis was above the WHO elimination threshold of 0.2% in six LGAs; in these LGAs support is required for trichiasis surgery services.